DNA Collection Guidelines
Purebred Dexter Cattle Association of North America (PDCA)
25979 Hwy EE, Prairie Home, MO 65068
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GUIDELINES FOR COLLECTING HAIR ROOT SAMPLES FOR DNA GENOTYPING


Items you will need:

Hair Sample Envelope  - (write animal I.D. , etc. on envelope before
collecting sample)

Application Form -
Chondrodysplasia (Bull Dog), Genetic Testing for Color, or Parentage Testing

Comb or Brush -  (Clean and rid of old hair prior to use for sampling)

Tape -  (to secure hair samples)

COLLECT HAIR FROM THE TIP OF THE TAIL (SWITCH). THE ROOT ENDS CONTAIN THE DNA. SAMPLES ARE TO BE PULLED NOT CLIPPED.

1. Clean the tail switch to remove any foreign material. Comb or brush the
tail to remove any dead hair. If needed, wash clean and rinse with water.
Wait for the tail to be completely dry. The sample must be free of urine or
manure. Dirty samples will not be processed. Contamination will make the
sample unfit for DNA testing.

2. Wrap approximately 5 strands of hair around a finger, about 2 inches away
from the skin, and give a sharp pull. Inspect the hair to ensure that the
follicles are attached. Hair strands without follicles do not contain DNA
and cannot be tested.

3. Repeat Step #2. until you have obtain 20 hair roots.

4. Place all of the hair roots at one end with the long strands pointing
straight down. Secure the hairs together with adhesive tape wrapped
approximately 1 inch from the follicles. Place the sample in the labeled
envelope and immediately seal to minimize contamination. Hair from only one
animal is to be placed in each envelope.

5. If doing more than one animal, WASH HANDS before starting on the next
animal or use a clean pair of surgical gloves. This will reduce the risk of
cross-contamination of DNA samples. Repeat Steps 1 to 4 for each animal.

This Site was created as a service by Rebecca Perez
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Purebred Dexter Cattle Association
Last modified: Saturday, May 10, 2008 08:06 PM
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